The promoter - probe vector phn117 in e . coli and phn127 in g " were further constructed by removing promoter while keeping sd sequence from phn115 . a teta / tetr bidirectional promoter fragment from pbr322 was respectively cloned into phn117 and phn127 and the resulted colonies were all fluorescent 并经插入pbr322上665bpteta tetr双向启动子片段后得到的转化子均发绿色荧光验证，实现了wtgfp在大肠杆菌和华癸中生根瘤菌中的组成型表达。
